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3 edition of Packings and stationary phases in chromatographic techniques found in the catalog.

Packings and stationary phases in chromatographic techniques

Packings and stationary phases in chromatographic techniques

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Published by M. Dekker in New York .
Written in English

    Subjects:
  • Packings (Chromatography),
  • Stationary phase (Chromatography),
  • Chromatographic analysis.

  • Edition Notes

    Includes bibliographies and index.

    Statementedited by Klaus K. Unger.
    SeriesChromatographic science ;, v. 47
    ContributionsUnger, K. K. 1936-
    Classifications
    LC ClassificationsQD79.C4 P33 1990
    The Physical Object
    Paginationviii, 836 p. :
    Number of Pages836
    ID Numbers
    Open LibraryOL2197170M
    ISBN 100824779401
    LC Control Number89016869

    The stationary phase normally consists of silica particles of diameter m, the surface pores having a diameter of to m. The surface area of the solid is therefore very high, and when coated with a liquid which can interact with the solute particles in the mobile phase, separation of a mixture becomes very efficient indeed.   Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it .

    Chromatography - Chromatography - Methods: Chromatographic methods are classified according to the following criteria: (1) geometry of the system, (2) mode of operation, (3) retention mechanism, and (4) phases involved. The mobile and stationary phases of chromatographic systems are arranged in such a way that migration is along a coordinate much longer than its width. General Principles of Chromatography Reversed Phase Chromatography (RPC) In this technique, one uses hydro-phobic interactions between the biological molecule and the ligand on the chromatographic support to obtain separation. RPC stationary phases differ from HIC packings in that they contain a higher density of hydrophobic ligand. Mobile phase.

    The planning and development of new chiral stationary phases (CSPs) for liquid chromatography (LC) are considered as continuous and evolutionary issues since the introduction of the first CSP in Packings and Stationary Phases in Chromatographic Techniques, edited by Klaus K. Unger Detection-Oriented Derivatization Techniques in Liquid Chromatography, edited by Henk Lingeman and Willy J. M. Underberg Chromatographic Analysis of .


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Packings and stationary phases in chromatographic techniques Download PDF EPUB FB2

Get this from a library. Packings and stationary phases in chromatographic techniques. [K K Unger;]. Read "Packings and stationary phases in chromatographic techniques.

Edited by Klaus K. Unger. Marcel Dekker: New York. 24 × 16 cm. ISBN 0‐‐‐1. $, Journal of Pharmaceutical Sciences" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Unger, K. Packings and stationary phases in chromatographic techniques / edited by Klaus K.

Unger M. Dekker New York Wikipedia Citation Please see Wikipedia's template documentation for further citation fields that may be required.

Journal of Chromatography, () Chromatographic Reviews Elsevier Science Publishers B.V., Amsterdam Printed in The Netherlands CHREV.

PACKINGS AND STATIONARY PHASES IN PREPARATIVE COLUMN LIQUID CHROMATOGRAPHY* K. UNGER* and R. JANZEN Institut fur Anorganische Chemie und Analytische Chemie, Johannes Gutenberg Cited by: PACKINGS AND STATIONARY PHASES IN CHROMATOGRAPHIC TECHNIQUES edited by Klaus K.

Unger, Marcel Dekker, $ (viii + pages) ISBN 0 1 Perhaps both the strength and the weakness of this book is its compre- hensiveness.

Packings and stationary phases in chromatographic techniques book somewhere in this book is a discussion of almost. My interest is for ion chromatography stationary phases. Some of the people when asked said it more art and magic rather than science. Of course the composition of slurries are trade secrets but is there some "science" i.e.

the physical properties requirements of packing compositions. Therefore, RFC is particularly suitable for soft stationary phases, which are prone to collapse at higher hydrostatic pressure.

Separation in RFC is achieved in the radial direction, so the chromatographic height is the radial thickness of the packing stationary phase. Accordingly, RFC offers fewer theoretical plates than an axial chromatography. Principles of Separation Techniques AB Molecular Characteristic Gas chromatography – Stationary phase: a film of a polymer or a wax.

The film must have a high boiling point – Mobile phase: gas (Helium is the usual carrier gas) solutes with the stationary phase.

Most HPLC packings. Chromatographic columns adhere by the old adage “like dissolves like” to achieve the separation of a complex mixture of chemicals. Columns are coated with a variety of stationary phases or chemical coatings on the column wall in capillary columns or on the inert column packing.

Based on the physical or chemical character of the stationary phase: This is especially followed in columnar chromatography wherein the stationary phase used has a specific character like being porous or charged. ♠ Size exclusion chromatography: Here the stationary phase has pores in its matrix.

When the molecules pass through, those with. Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.

The various constituents of the mixture. Gas chromatography is an important variation that you should know about. Instead of passing a liquid over the stationary phase, an inert gas moves over the stationary phase.

The inert gas may be helium or nitrogen. The equilibrium here is between compounds absorbed onto the stationary phase and compounds moving in the gas phase.

In normal phase chromatography, the stationary bed is strongly polar in nature (e.g., silica gel), and the mobile phase is nonpolar (such as n-hexane or tetrahydrofuran).Polar samples are thus retained on the polar surface of the column packing longer than less polar materials. Reversed-phase chromatography is the inverse of this.

The stationary bed is nonpolar (hydrophobic) in nature, while. Agarose-gel chromatography is used for the purifi-cation of RNA, DNA particles, and viruses [4].

Stationary phase in chromatography, is a solid phase or a liquid phase coated on the surface of a solid phase. Mobile phase flowing over the station-ary phase is a gaseous or liquid phase.

If mobile phase is liquid it is termed as liquid chromatogra. The stationary phase usually consists of solid beads packed into a column adhered to a capillary tube. A more useful mobile phase in proteomics is one that is in a liquid state.

All of the techniques discussed in this chapter involve liquid chromatography. In this technique, there is traditionally a partition equilibrium between a solid.

For separation of polar compounds or complex mixtures by normal (NP) or reversed phase liquid chromatography (RP-HPLC) and/or electromigration techniques, it is. Overall Chromatographic System Optimization Conflicts During Optimization of Chromatographic Systems Stationary Phase Gradients References 5 Process Concepts Malte Kaspereit and Henner Schmidt-Traub.

Discontinuous Processes Isocratic Operation Gradient Chromatography phase than the solutes that prefer the stationary phase. As the solutes move through the stationary phase they separate. This is called chromatographic development. How it works In all chromatography there is a mobile phase and a stationary phase.

The stationary phase is the phase that doesn't move and the mobile phase is the phase that does move. In all forms of chromatography, samples equilibrate between stationary and mobile phases. In almost all applications of TLC, the stationary phase is a silica or alumina adsorbent and the mobile phase is an organic solvent or solvent mixture (the "eluent") that rises up the plate (equation 3).

Several recent comprehensive reviews and book articles summarize chromatographic [] and electrophoretic methods [] for chiral separa-tion in general. High performance liquid chromatography (HPLC) HPLC can be used to separate enantiomers either indirectly with chiral derivatization reagents or directly with chiral stationary phases or chiral.

Chromatography Theory. Chromatography is a method of separating molecule. The method takes advantage of differences between a mobile phase and a stationary phase to separate the different components in a mixture.

The target molecules can interact with the stationary phase based on characteristics such as charge, size, and hydrophobicity.2. Reversed phase chromatography: Reversed phase HPLC (RP-HPLC) consists of a non-polar stationary phase and a moderately polar mobile phase.

One common stationary phase is a silica which has been treated with RMe 2 SiCl, where R is a straight chain alkyl group such as C 18 H 37 or C 8 H The retention time is, therefore, longer for.Gas chromatography Science Class 11 Chemistry (India) Organic chemistry - Some basic principles and techniques Methods of purification of organic compounds Principles of chromatography.